《植物生理学报》 2015, 51(10): 1757-1763

番茄不孕病毒(TAV)脱除对‘怀白菊’活性氧代谢的影响

赵喜亭^{1, 2,3,*}, 田莹¹, 周颖媛¹, 范子建¹, 朱玉婷¹, 蒋丽微¹, 李明军^{1, 2,3,*}

¹河南师范大学生命科学学院, 河南新乡453007; ²绿色药材生物技术河南省工程实验室, 河南新乡453007; ³河南省高校道地中药材保育及利用工程技术研究中心, 河南新乡453007

通信作者：赵喜亭；E-mail: zhaoxt0411@126.com,limingjun2002@263.net；Tel: 18237391085, 13613731041

摘　要：

以前期获得的脱除番茄不孕病毒(TAV)的‘怀白菊’试管苗为试材, 从同工酶谱和DNA分子简单序列间重复(ISSR)多态性方面分析其遗传稳定性, 在此基础上, 对其继代培养过程中叶片中的活性氧代谢进行了研究。结果表明, TAV脱除没有改变‘怀白菊’叶片中SOD、POD同工酶谱带及ISSR扩增条带的数目, 但相应同工酶谱带亮度增强; 也没有改变‘怀白菊’试管苗继代培养过程中膜脂过氧化相关指标(O₂ ¯•生成速率及H₂O₂和MDA含量)和保护系统相关指标(保护酶SOD、POD、CAT、APX和GR, 抗氧化物质GSH和AsA)的变化趋势, 但却显著降低了相同时间点膜脂过氧化相关指标的绝对值, 降幅最大值超过30% (O₂ ¯• 生成速率、MDA含量), 显著增加了保护酶活性及抗氧化物质的含量, 增幅最大值超过了50% (APX活力、GSH含量)。以上说明, TAV脱除不仅没有改变‘怀白菊’的遗传稳定性, 而且降低了其活性氧代谢水平, 提高了其抗氧化能力。

关键词：‘怀白菊’; 番茄不孕病毒; 脱毒; 遗传稳定性; 活性氧代谢

收稿：2015-06-19   修定：2015-09-02

资助：国家自然科学基金(31372105)、中医药行业科研专项子课题(201407005-08)、中国博士后科学基金(2011M500457)、中央高校基本科研业务费专项资金(2011JS076)、河南省高校科技创新团队支持计划基金(15IRTSTHN020)和河南省创新型科技人才队伍建设工程基金(C20130037)。

Effect of TAV-Free on Active Oxygen Metabolism of Chrysanthemum morifolium ‘Huaibai’

ZHAO Xi-Ting^{1, 2,3,*}, TIAN Ying¹, ZHOU Ying-Yuan¹, FAN Zi-Jian¹, ZHU Yu-Ting¹, JIANG Li-Wei¹, LI Ming-Jun^{1, 2,3,*}

¹College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China; ²Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan Province, Xinxiang, Henan 453007, China; ³Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Xinxiang, Henan 453007, China

Corresponding author: ZHAO Xi-Ting; E-mail: zhaoxt0411@126.com,limingjun2002@263.net; Tel: 18237391085, 13613731041

Abstract:

Tomato aspermy virus (TAV)-free Chrysanthemum morifolium ‘Huaibai’ test-tube plantlets were used as experimental materials. In this study, genetic stability of TAV-free ‘Huaibai’ test-tube plantlets was analyzed from isozyme zymogram and DNA molecular ISSR polymorphism. Based on this, active oxygen metabolism of TAV-free ‘Huaibai’ plantlets had been studied during subculture. The results showed that: TAV-free didn’t change the number of SOD isozyme and POD isozyme bands but the band brightness were brighter than the virus-carrying plantlets, and also didn’t change the number and brightness of ISSR amplification bands. So TAV-free maintained its genetic stability and increased the activities of SOD and POD. TAV-free also didn’t change the trend of lipid peroxidation related indexes (O₂ ¯• produce rate, the MDA and H₂O₂ contents) and protection system related indexes (protective enzymes SOD, POD, CAT, APX and GR activities; antioxidants GSH and AsA contents) during subculture, but significantly reduced the absolute value of lipid peroxidation related indexes and significantly increased activities of protective enzyme and the contents of antioxidants at the same time of subculture. The maximum decrease of more than 30% (O₂ ¯• produce rate and MDA content) and the maximum increase of more than 50% (APX activity and GSH content) were gotten in the leaves of TAV-free plantlets. In conclusion, TAV-free didn’t only change genetic stability of Chrysanthemum morifolium ‘Huaibai’, but also reduced the active oxygen metabolism and improved its antioxidant capacity.

Key words: Chrysanthemum morifolium ‘Huaibai’; tomato aspermy virus; virus-free; genetic stability; active oxygen metabolism